RESULTS
: The final submitted Research report says as follows:
Many herbal formulations from medicinal plants that will protect
the liver from or reverse the hepatotoxicity are sought after by
the common man. We came across a Unani formulation, Ashshifa-1000
that is claimed to be hepatoprotective. The biochemical and histopathological
examination of the liver of mice administered with CCL4 alone and
co-administered with Ashshifa-1000, in this investigation, confirmed
that Ashshifa-1000 is hepatoprotective.
Ashshifa-1000 has been found to contain antioxidants like riboflavin,
tocopherol, ascorbic acid and flavonoids. Further it has been proved
to be antimutagenic and anticarcinogenic. It is non-toxic and does
not have any side effect. Co-administration of Ashshifa-1000 with
CCL4 has caused lesser magnitude of liver damage in mice. The liver
damage marker enzymes AST and ALT have been elevated significantly
in CCL4 treated animals. Also, liver ALP and LDH enzymes and serum
bilirubin were increased. But the liver glycogen content was reduced
in these mice.
But upon treatment with Ashshifa-1000, the raised levels of liver
AST, ALT, ALP, LDH and serum bilirubin were brought down. The liver
glycogen content increased. However, these had not reached the levels
of the normal control mice.
Histopathological examination of the liver showed extensive mild
peripheral steatosis in mice treated with CCL4 alone. The animals
treated with Ashshifa-1000 (cotreated) showed less extensive damage.
Thus alterations of liver enzyme levels in hepatotoxicity were confirmed
by histopathological studies of the liver.
The normai liver architecture in the treated with Ashshifa-1000
prior to and after CC14 treatment has confirmed that this herbal
product is hepatoprotective.
REPORT BY :
Dr. S. Saroja, Professor & Head, Dr. P.R. Padma, Reader, Ms.
S. Gayathri Devi, Lecturer, and Ms. A. Lalitha, Research Scholar,
Department of Biochemistry, Avinashilingam University. |
